Biochemistry and Biophysics Facility

The biochemistry and biophysics (B&B) facility is dedicated to the production, functionalization and analysis of recombinant proteins, as well as the characterization of protein-protein and protein-lipid interactions.

 

Proteins are produced in various organisms (e.g. bacteria, yeast, mammalian cells) and purified/functionalized with an ÄKTA Pure 25M system (GE Healthcare) equipped with various modes of liquid chromatography. Purified proteins are analyzed by electrophoresis, mass spectrometry and dynamic light scattering (Zetasizer Nano ZSP, Malvern Instrument) and their interaction with lipids or other proteins are characterized by preparative ultracentrifugation (e.g. liposome co-floatation assay) and spectroscopy (2 plates readers: Mithras LB 940, Berthold and Envision, Perkin Elmer). We also plan to acquire a circular dichroism (CD) system for structural studies and an isothermal titration calorimetry (ITC) system for thermodynamics studies.

 

Contacts

 

Platform facilitator:

David Tareste (e-mail; phone: +33 1 40 78 92 49)

 

Research engineer:

Joanna Lipecka (e-mail ; phone: +33 1 40 78 92 25)

 

Production, functionalization and analysis of recombinant proteins

 

Characterization of molecular interactions